Alfred Pingoud Bücher

Die Nordrhein-Westfälische Akademie der Wissenschaften und der Künste ist eine Vereinigung der führenden Forscherinnen und Forscher des Landes. Sie wurde 1970 als Nachfolgeeinrichtung der Arbeitsgemeinschaft für Forschung des Landes Nordrhein-Westfalen gegründet. Die Akademie ist in drei wissenschaftliche Klassen für Geisteswissenschaften, für Naturwissenschaften und Medizin sowie für Ingenieur- und Wirtschaftswissenschaften und in eine Klasse der Künste gegliedert. Mit Publikationen zu den wissenschaftlichen Vorträgen in den Klassensitzungen, zu öffentlichen Veranstaltungen und Symposien will die Akademie die Fach- und allgemeine Öffentlichkeit über die Arbeiten der Akademie und ihrer Forschungsstellen informieren.

Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.

This book gives a profound overview on the relevant biochemical techniques. Moreover, it refers to laboratory equipment and safety aspects and explains how to obtain relevant biochemical information. It provides an introduction into physical-chemical processes and mathematical methods required for the interpretation of data. Principles of expensive instrumental analysis are also explained and a presentation of safety considerations and regulatory issues according to international requirements is given. With its practical approach the book is not only highly useful for professionals - laboratory technicians and scientists - but also for students. Special feature: a CD-ROM on quantitative analysis of biochemical experiments! „... An ideal how-to for those working in biochemistry.“ CHEMIE in unserer Zeit „... and anyone working in a biochemical laboratory will find it useful. Strongly recommended.“ Laboratory News