Lester Packer Bücher

A NATO Advanced Study Institute on „Molecular Mechanisms of Transcellular Signaling: from the Membrane to the Gene“ was held on the Island of Spetsai, Greece, from August 15- 27, 1994. The aim of this Institute was to bring together researchers in the field of signal transduction mechanisms, transcription factors and gene regulation with those actively involved in studies on the implications of oxygen radicals and antioxidant defence mechanisms for cell function. As diverse as these fields may be, the emergence of their interconnection during the course of the Institute was an eye-opener for students and lecturers alike. 2 Presentations and discussions focussed on the role of Ca +, G-proteins, protein kinase C and phospholipases in signaling mechanisms. These broad principles were extended to transcription factors and gene regulation with an emphasis on the steroid hormone receptor superfamily and NFKB. Basic principles of free radical formation and antioxidant action (vitamin E and C) were presented and discussed in connection with effects on signaling pathways. This book present the content of the major lectures and a selection of the most relevant posters. These proceedings offer a comprehensive account of the most important topics discussed at the Institute. The book is intended to make the proceedings accessible to a large audience.

In recent years, cyanobacteria have become the focus of intense study. As simple bacteria, they would seem to be the obvious organisms of choice for the study of such fundamental processes as oxygen-evolving photosynthesis and nitrogen fixation. This book will prove useful to investigators with little previous experience with cyanobacteria as well as to those who wish to change the emphasis of their research.

The methods in Volumes 189 and 190 apply to the use of retinoids in basic research in molecular, cellular, and development biology and in clinical medicine.

This volume contains papers selected from the twenty-four Methods in Enzymology volumes on Biomembranes . The articles were chosen based on their impact on the field and how well the methods they cover have withstood the test of time. They represent a milestone in our understanding by the giants in the field.Key Features* Purification, functional characterization, reconstitution, and organization* Transmembrane and associated proteins* Membrane lipids and lipoproteins* Bioenergetics and transport* Biogenesis, targeting, and assembly

This volume and its companion, volume 214 of Methods in Enzymology, present a comprehensive, state-of-the-art compilation of the molecular and cellular methodology needed for pursuing research with carotenoids.

Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 234 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. Key Features * Generation, detection, and characterization of oxygen radicals, chemistry, biochemistry, and intermediate states of reductio* Isolation, characterization, and assay of enzymes or substrates involved in formation or removal of oxygen radical * Methods for assessing molecular, cell, and tissue damage; assays and repair of oxidative damage

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Characterization of membranes and membrane components; Isolation of selected membrane components; Model membranes; Isolation and culture of cells