Handbook of RNA biochemistry

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This all-in-one compendium of current laboratory techniques for the study of RNA brings together numerous novel methods that were hitherto only available through the primary literature. The editors - themselves experienced RNA researchers - have gathered expert authors from the US and Europe for the ultimate in everyday working knowledge. In more than 300 detailed protocols, all the techniques covered are treated with the user in mind, including practical advice and an invaluable "troubleshooting" section. The broad scope of the book allows the side-by-side treatment of specialized methods with basic biochemical techniques for the isolation, characterization and functional analysis of RNA. All pertinent laboratory methods are presented in detail, including * RNA synthesis, modification and ligation * Sequence and structure determination of RNA * Isolation and purification of ribonucleoproteins * RNA crystallization * Single molecule studies on RNA * RNA imaging in vivo * RNA bioinformatics * Modeling and prediction of RNA structure * RNA microarrays * Aptamer libraries and SELEX technology * RNA interference "The book is helpful for molecular biologists, biochemists and biotechnologists and of interest to every researcher experimenting with RNA." - New Drugs "A wonderful guide to successful experimentation in the field of RNA research." - Engineering in Life Sciences