Modern Methods in Protein Chemistry/Review Articles, including those from an International Conference held in Bielefeld, F. R. of Germany, June 1–2, 1984

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This comprehensive resource covers a range of modern micromethods in protein analysis and purification. It begins with a review of chromogenic peptide substrates for serine proteases and explores immobilized metal ion affinity chromatography, highlighting its effectiveness in protein purification. The use of synthetic dyes for affinity chromatography is discussed, along with fluorescence-activated cell sorting for isolating genetic variants to analyze protein structure and function. The text details the FPLC system for fast, high-resolution protein separations and methods for amino acid analysis via high-performance liquid chromatography with pre-column derivatization. It examines high-performance ion-exchange chromatography's potential in protein separations and the separation of cyanogen bromide peptides from NADPH-cytochrome P-450 reductase using HPLC on wide pore columns. Immunoassays for peptides and proteins, chemical modification techniques, and cross-linking reagents are also covered. The book includes a micro-analysis of polypeptide structures with color reagents and a modular system for microsequencing proteins and synthesizing oligonucleotides and peptides. Additionally, it discusses on-line detection of amino acid derivatives from automatic Edman degradation, programmable electronic control of analytical devices, and improved isocratic separation techniques. The resource concludes with applications of computer